CHARACTERIZATION , IN VITRO ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITY OF FUNGAL ENDOPHYTIC EXTRACTS OF ANDROGRAPHIS PANICULATA LEAVES IN CCL Original Article 4 INDUCED HEPATOTOXICITY
By: Puri, Smita K
.
Contributor(s): Habbu, Prasanna V.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(1).Description: 44-54p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
To isolate and characterize the endophytic fungi from the leaves of
Andrographis paniculata
for free radical scavenging antioxidant
and
hepatopr
otective activity against
CCl
4
Method
s:
Two fungal
endophytes,
APLF
-3 (
Andrographis
paniculata
leaf fungi
-3) and APLF
-4 (
Andrographis
paniculata
leaf fungi
-4)
were isolated
from
leaves of
Andrographis
paniculata
to get chloroform (A3C, A4C), ethyl acetate (A3EA, A4EA) and n butanol (A3nB, A4nB) extracts. rDNA
sequencing by PCR technique was carried out for identification of APLF
-3 and APLF
-4. All the APLF
-3 and APLF
-4 extracts were assayed for
in vitro
free radical scavenging activity against 2, 2
-diphenyl
-1-picrylhydrazyl (DPPH), hydroxyl radical and redu
cing power. Then, A4EA and A4nB were
screened for hepatoprotective activity against CCl
induced hepatotoxicity
4
Result
s:
The endophytic fungi, APLF
-3 and
APLF
-4, were identified as
Phyllosticta sp. ZLY
-2010 isolate M13
and
Aspergillus
tubingensis
strain Cs/7/2
respectively based on their morphological and molecular characterization. A4EA and A4nB showed significant
in vitro
free radical scavenging
activity as compared to other extracts.
A4EA and A4nB (50 mg/kg
and
100 mg/kg) reversed the increased serum biochemical parameters as
compared to CCl
induced hepatotoxicity at 50 mg/kg and
100 mg/kg doses.
4
Conclusio
n:
These findings suggested that the extracts (A4EA and A4nB) obtained from
endophytic fungi APLF
-4 contributed towards
hepatoprotective activity.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020935 |
Objective:
To isolate and characterize the endophytic fungi from the leaves of
Andrographis paniculata
for free radical scavenging antioxidant
and
hepatopr
otective activity against
CCl
4
Method
s:
Two fungal
endophytes,
APLF
-3 (
Andrographis
paniculata
leaf fungi
-3) and APLF
-4 (
Andrographis
paniculata
leaf fungi
-4)
were isolated
from
leaves of
Andrographis
paniculata
to get chloroform (A3C, A4C), ethyl acetate (A3EA, A4EA) and n butanol (A3nB, A4nB) extracts. rDNA
sequencing by PCR technique was carried out for identification of APLF
-3 and APLF
-4. All the APLF
-3 and APLF
-4 extracts were assayed for
in vitro
free radical scavenging activity against 2, 2
-diphenyl
-1-picrylhydrazyl (DPPH), hydroxyl radical and redu
cing power. Then, A4EA and A4nB were
screened for hepatoprotective activity against CCl
induced hepatotoxicity
4
Result
s:
The endophytic fungi, APLF
-3 and
APLF
-4, were identified as
Phyllosticta sp. ZLY
-2010 isolate M13
and
Aspergillus
tubingensis
strain Cs/7/2
respectively based on their morphological and molecular characterization. A4EA and A4nB showed significant
in vitro
free radical scavenging
activity as compared to other extracts.
A4EA and A4nB (50 mg/kg
and
100 mg/kg) reversed the increased serum biochemical parameters as
compared to CCl
induced hepatotoxicity at 50 mg/kg and
100 mg/kg doses.
4
Conclusio
n:
These findings suggested that the extracts (A4EA and A4nB) obtained from
endophytic fungi APLF
-4 contributed towards
hepatoprotective activity.
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